Integration of localized electrically deciphered epigenetic profiles for enhanced stem cell therapy

One fully funded Frederick E Hopper full-time PhD scholarship is available in the School of Dentistry for a start in October 2024. The scholarship will cover UK tuition fees and attract a stipend of £19,237, subject to satisfactory progress.

The award is open to full-time or part-time candidates (UK only) who meet the eligibility for a place on a PhD degree at the School of Dentistry. This project would suit a student with a microbiology or molecular biology background.

Candidates whose first language is not English must provide evidence that their English language is sufficient to meet the specific demands of their study.

Project Summary

The global regenerative medicine market size is projected to be US$197.08 billion by 2030. However, the effectiveness of these therapies are big challenge for clinical translation. Following the dawn of AI, real-time sensing and monitoring have played a crucial role in enhancing regenerative medicine technologies. This proposal seeks to combine the expertise in stem cells (Yang), biomaterials (Tronci), microbiology (Do) and bioelectrical engineering (Gao, RWTH, Germany) and aims to employ a novel cutting-edge approach in dynamic real-time sensing and monitoring with biohybrid electronics for advancing epigenetic control in stem cell differentiation, which improve the tissue engineering efficacy. This unique approach leverages our expertise in epigenetic modification [1,2] and biohybrid electronic sensors [3,4] to decipher the mechanism of human dental pulp stem cells (hDPSCs) functionality on 3D hydrogel scaffolds with photodynamic infection control capability [5]. This offers unprecedented insight into cellular dynamics, facilitates the identification of regulatory factors and critical time points, enables personalized treatment strategies, enhances drug screening, and promotes translation to clinical applications for improved bone regeneration and patient care. HDPSCs are pre-treated with histone deacetylase inhibitor (HDACi) to enhance stem cells’ cellular responses. The micro/nano-electronic & fabrication technologies allow non-invasive multidimensional cell-sensor coupling and hybrid biosignal integration so that localized electric stimulation and signal recording will be delivered to guide stem cell differentiation on/in 3D hydrogel scaffolds in real-time, which will lead to a readable and programmable epigenetic control system, offering multidimensional tools to promote bespoke stem cell therapy, infection diagnosis and staining-free photodynamic infection control. 

Aims & Objectives:

This project aims to combine epigenetic modification and a novel cutting-edge approach in dynamic real-time sensing and monitoring with biohybrid electronics to decipher the mechanism of stem cell functionality for enhancing the efficacy of bone tissue regeneration. 

• Isolation and characterisation of human dental pulp stem cell from fresh sound human tooth.
• The effect of epigenetic modification on hDPSCs osteogenesis in monolayer and 3D culture (hydrogel).
• Integration of biohybrid electronic sensing system for hDPSCs study: validation and optimisation.
• The effect of photodynamic therapy on the infection control capability of 3D hydrogel scaffolds.
• Real-time monitoring of cellular/bacterial responses: sensing, stimulation, and differentiation guidance.
• Epigenetic analysis and epi-electric signal correlation.
• In In vivo testing uses animal models.

Eligibility

Applicants to this scholarship should normally have an Undergraduate degree of 2:1 or above (or international equivalent) in a relevant subject area. A Masters degree is desirable but not essential.

If English is not your first language, you must provide evidence that you meet the University’s minimum English language requirements. The minimum English language entry requirement for the Faculty of Medicine & Health is an IELTS of 6.5 overall with at least 6.0 in each component or equivalent. The test must be dated within two years of the start date of the course in order to be valid.

How to Apply

To apply for this scholarship opportunity applicants should complete an online application form and attach the following documentation to support their application. 

• A full academic CV
• Degree certificate and transcripts of marks
• Evidence that you meet the University’s minimum English language requirements (if applicable)

To help us identify that you are applying for this scholarship project please ensure you provide the following information on your application form;

• Select PhD in Dentistry as your programme of study
• Give the full project title and name the supervisors listed in this advert
• For source of funding please state you are applying for a Frederick E Hopper Scholarship

For further information please contact the Faculty PGR Admissions Team at fmhpgradmissions@leeds.ac.uk