PhD position (DNA Repair & Genome Stability): DNA damage signaling, DNA repair & Cancer (m/f/d)

18 Sep 2023
Job Info

Organisation/Firm

Worldwide PhD Programme (IPP) Mainz

Division

Institute of Molecular Biology

Analysis Subject

Organic sciences

Researcher Profile

First Stage Researcher (R1)

Nation

Germany

Software Deadline

8 Nov 2023 – 12:00 (Europe/Berlin)

Sort of Contract

Short-term

Job Standing

Full-time

Is the job funded via the EU Analysis Framework Programme?

Not funded by an EU programme

Is the Job associated to workers place inside a Analysis Infrastructure?

No

Supply Description

Considering of doing all of your PhD within the Life Sciences? The Worldwide PhD Programme  (IPP) Mainz is providing gifted college students the possibility to work on the reducing fringe of analysis. As an IPP PhD pupil, you’ll be part of a group of outstanding scientists engaged on various subjects starting from how organisms age or how our DNA is repaired, to how epigenetics regulates mobile id or neural reminiscence.

 

Actions and obligations:

Within the subject of “DNA Restore & Genome Stability”, the analysis group of Thomas Hofmann gives the next PhD undertaking:

DNA injury is a continuing risk to genome stability, and devoted DNA injury signaling and DNA restore play a basic position in counteracting ageing and most cancers. DNA double-strand breaks (DSBs) are harmful DNA lesions that are repaired by high-fidelity homologous recombination (HR) restore or the error-prone non-homologous end-joining (NHEJ) pathway. HR restore is incessantly compromised in most cancers cells. DSBs originate from exogenous and endogenous sources, and specifically extremely proliferative most cancers cells set off the replication stress response, which prompts the DNA injury signaling cascade. Our unpublished work recognized a novel BRCA1-associated DNA injury signaling module with a vital position within the restore of extrinsic DSBs by regulating HR restore. Apparently, our outcomes point out exercise of this novel BRCA1-associated signaling module throughout replication stress, suggesting a task within the replication stress response evoked by stalled and/or damaged DNA replication forks. This PhD undertaking is geared toward unraveling the operate of a novel BRCA1-associated signaling module in replication stress.

PhD undertaking: DNA injury signaling, DNA restore & Most cancers

This PhD undertaking is embedded within the SFB 1361 consortium and geared toward elucidating the mechanisms interconnecting DNA injury signaling, DNA restore and cell destiny selection. Particularly, the signaling modules and equipment intertwining DNA double-strand break (DSB) restore upon replication stress can be analyzed from perspective of a novel BRCA1-associated signaling module found in our lab. To find out the operate of the BRCA1-associated module at arrested and damaged replication forks, a broad spectrum of strategies from cell biology, molecular biology, biochemistry and molecular genetics can be used. This extremely interactive undertaking can be carried out in shut collaboration with quite a few Z-projects and members of the SFB 1361. The work program consists of mass spectrometry-based replication fork-interactome evaluation (iPOND), genome-wide mapping of the novel BRCA1-associated module utilizing Lower&Tag seq, genome-wide evaluation of DNA breaks utilizing GLOE-seq and sBLISS, DNA fiber assays, confocal microscopy, circulate cytometry, Crisp/Cas9-mediated gene enhancing, dTAG-mediated focused degradation and protein-protein interplay evaluation. The next questions can be addressed: 1. Is the BRCA1-associated module related to stalled or damaged replication forks, and the way does act throughout replication stress? 2. What are the mobile (cell demise, senescence) and molecular (DNA injury signaling, DNA restore & DNA replication fork) penalties of perturbing this module? 3. Does pharmacological or genetic perturbation of the module sensitize most cancers cells to anti-cancer therapies?

If you’re on this undertaking, please choose Hofmann as your group desire within the IPP utility platform.

 

What we provide:

• Thrilling, interdisciplinary tasks in a completely worldwide atmosphere, with English as our working language
• Superior coaching in scientific strategies {and professional} abilities
• Entry to our state-of-the-art Core Amenities and their technical experience
• Absolutely funded positions with financing till the completion of your thesis
• A vigorous group of greater than 190 PhD college students from 44 totally different international locations

 

Necessities:

Are you an formidable, younger scientist seeking to push the boundaries of science whereas interacting with colleagues from a number of disciplines and cultures? Then the IPP is your alternative to offer your scientific profession a flying begin!

 

All you want is:

• Grasp or equal
• Interactive character & good command of English
• 2 letters of reference

 

For extra particulars on the tasks provided and the way to apply by way of our on-line kind, please go to https://www.imb.de/phd

The deadline for functions is 8 November 2023. Interviews will happen at IMB in Mainz on 22-24 January 2024.

Beginning date: 1 March 2024 – 1 August 2024

 

Necessities

Analysis Subject

Organic sciences

Training Stage

Grasp Diploma or equal

Further Info
Work Location(s)

Variety of gives accessible

1

Firm/Institute

Worldwide PhD Programme (IPP) Mainz

Nation

Germany

Metropolis

Mainz

Geofield

The place to use

Web site

https://imb.de/phd/apply

Contact

Metropolis

Mainz

Web site

http://www.imb-mainz.de/PhD

Road

Ackermannweg 4

Postal Code

55128

STATUS: EXPIRED