Research Associate

Job id: 086767. Wage: £43,205 every year, together with London Weighting Allowance.

Posted: 22 March 2024. Cut-off date: 01 April 2024.

Enterprise unit: School of Life Sciences & Medication. Division: Complete Most cancers Centre.

Contact particulars:Elinor Sawyer. [email protected]

Location: Man’s Campus. Class: Analysis.

Job description

This one-year submit funded by Most cancers Analysis UK is specializing in the evaluation of the tumour immune microenvironment in ductal carcinoma in situ (DCIS) and invasive breast most cancers utilizing spatial transcriptomics, with the goal of figuring out a signature of radiation resistance in breast most cancers.

The submit holder can be primarily based within the Guys Most cancers Most cancers Centre (Innovation Hub), Faculty of Most cancers & Pharmaceutical Sciences, Kings Faculty London and be a part of the Molecular Breast Most cancers Genetics Group led by Professor Elinor Sawyer.

Radiotherapy is routinely used within the therapy of breast most cancers to scale back the danger of recurrence. Nonetheless, regardless of radiotherapy some sufferers nonetheless expertise native recurrences, and that is related to worse long-term outcomes.

The postholder will give attention to the evaluation of the tumour microenvironment in two impartial samples units, one in every of DCIS and one in every of invasive breast most cancers. All samples have been handled with surgical procedure and postoperative radiotherapy and have long run end result information on recurrence. Nanostring GeoMx Digital Spatial Profiler (DSP) can be used to evaluate the entire transcriptome of distinct organic compartments throughout the tumour microenvironment – immune, stromal and tumour.

Tissue microarrays have already been from the DCIS samples and can should be produced from the set of invasive samples. The group of Professor Grigoriadis (additionally primarily based within the Innovation Hub, Guys Most cancers Centre) runs the nanoString GeoMx DSP facility and pipelines to analyse core biopsies, resections and tissue microarrays are established already established.

It is a one-year submit to generate preliminary information with the hope of securing additional funding to increase the evaluation to different breast most cancers samples units. The submit holder can be anticipated to:

• Choose samples for evaluation
• Carry out spatial transcriptomics utilizing the Nanostring GeoMx spatial transcriptomics platform
• Be concerned within the evaluation of the spatial transcriptomics with the help of a bioinformatician

This submit can be supplied on a fixed-term contract till 30 April 2025.

It is a full-time submit – 100% full time equal.

Key duties

• Number of samples for evaluation
• Performing spatial transcriptomics utilizing the Nanostring GeoMx spatial transcriptomics platform
• Evaluation of the spatial transcriptomics with the help of a bioinformatician

The above checklist of duties might not be exhaustive, and the submit holder can be required to undertake such duties and duties as might fairly be anticipated throughout the scope and grading of the submit.

Abilities, information, and expertise

Important standards  

PhD in Organic Sciences

Information of most cancers genetics and obtainable assets

Database administration

Expertise of working with FFPE tissue specimens

Competent within the interpretation and technical validation of outcomes

Competent in using customary statistical evaluation packages

Fascinating standards 

Expertise of Nanostring GeoMx spatial transcriptomics platform

* Please notice that it is a PhD stage function however candidates who’ve submitted their thesis and are awaiting award of their PhDs can be thought-about. In these circumstances the appointment can be made at Grade 5, backbone level 30 with the title of Analysis Assistant. Upon affirmation of the award of the PhD, the job title will turn out to be Analysis Affiliate and the wage will enhance to Grade 6.

Interview date is scheduled for fifteenth April.

This submit is topic to Occupational Well being clearance.