Research Associate

Job id: 086767. Wage: £43,205 each year, together with London Weighting Allowance.

Posted: 22 March 2024. Deadline: 01 April 2024.

Enterprise unit: College of Life Sciences & Drugs. Division: Complete Most cancers Centre.

Contact particulars:Elinor Sawyer. [email protected]

Location: Man’s Campus. Class: Analysis.

Job description

This one-year put up funded by Most cancers Analysis UK is specializing in the evaluation of the tumour immune microenvironment in ductal carcinoma in situ (DCIS) and invasive breast most cancers utilizing spatial transcriptomics, with the goal of figuring out a signature of radiation resistance in breast most cancers.

The put up holder can be based mostly within the Guys Most cancers Most cancers Centre (Innovation Hub), College of Most cancers & Pharmaceutical Sciences, Kings Faculty London and be a part of the Molecular Breast Most cancers Genetics Group led by Professor Elinor Sawyer.

Radiotherapy is routinely used within the remedy of breast most cancers to cut back the chance of recurrence. Nevertheless, regardless of radiotherapy some sufferers nonetheless expertise native recurrences, and that is related to worse long-term outcomes.

The postholder will concentrate on the evaluation of the tumour microenvironment in two unbiased samples units, one among DCIS and one among invasive breast most cancers. All samples have been handled with surgical procedure and postoperative radiotherapy and have long run final result information on recurrence. Nanostring GeoMx Digital Spatial Profiler (DSP) can be used to evaluate the entire transcriptome of distinct organic compartments inside the tumour microenvironment – immune, stromal and tumour.

Tissue microarrays have already been from the DCIS samples and can must be constructed from the set of invasive samples. The group of Professor Grigoriadis (additionally based mostly within the Innovation Hub, Guys Most cancers Centre) runs the nanoString GeoMx DSP facility and pipelines to analyse core biopsies, resections and tissue microarrays are established already established.

It is a one-year put up to generate preliminary information with the hope of securing additional funding to develop the evaluation to different breast most cancers samples units. The put up holder can be anticipated to:

• Choose samples for evaluation
• Carry out spatial transcriptomics utilizing the Nanostring GeoMx spatial transcriptomics platform
• Be concerned within the evaluation of the spatial transcriptomics with the help of a bioinformatician

This put up can be supplied on a fixed-term contract till 30 April 2025.

It is a full-time put up – 100% full time equal.

Key duties

• Choice of samples for evaluation  
• Performing spatial transcriptomics utilizing the Nanostring GeoMx spatial transcriptomics platform 
• Evaluation of the spatial transcriptomics with the help of a bioinformatician 

The above listing of duties might not be exhaustive, and the put up holder can be required to undertake such duties and duties as could moderately be anticipated inside the scope and grading of the put up.

Abilities, information, and expertise

Important standards  

PhD in Organic Sciences 

Data of most cancers genetics and accessible sources 

Database administration 

Expertise of working with FFPE tissue specimens 

Competent within the interpretation and technical validation of outcomes 

Competent in using normal statistical evaluation packages 

Fascinating standards 

Expertise of Nanostring GeoMx spatial transcriptomics platform 

* Please observe that this can be a PhD stage position however candidates who’ve submitted their thesis and are awaiting award of their PhDs can be thought-about. In these circumstances the appointment can be made at Grade 5, backbone level 30 with the title of Analysis Assistant. Upon affirmation of the award of the PhD, the job title will develop into Analysis Affiliate and the wage will improve to Grade 6.

Interview date is scheduled for fifteenth April.

This put up is topic to Occupational Well being clearance.