Research Technician
University of Leeds
Are you looking for your next challenge? Are you interested in using model systems to understand human disease? Can you provide technical support to research projects and do you have laboratory experience which includes skills in cell culture as well as molecular, biochemical, tissue analysis?We are looking for a proficient technician to join the Malignant Hyperthermia (MH) research group. You will be part of a larger University and NHS team. MH is a pharmacogenetic disorder of skeletal muscle that is triggered by the commonly used inhalational anesthetics (e.g. isoflurane, sevoflurane) or the muscle relaxant succinylcholine. It presents predominantly in apparently healthy children and young adults undergoing general anesthesia as a syndrome of hypermetabolism, hyperthermia and rhabdomyolysis that is fatal unless aggressive treatment with dantrolene is rapidly instituted. The project will investigate a novel knock-in Stac3 mouse model using a range of techniques. Understanding how Stac3 affects skeletal muscle function will not only allow us to facilitate diagnosis, and will allow better understanding of abnormal molecular and phenotypic responses.You will provide support for the research project, and be primarily responsible for the primary cell cultures. A substantial part of the role will be undertaken on primary cells as the post requires initial expansion of the cells to generate independent pure myoblast cultures from single animals of different genotypes of both sexes to produce n=18 cell lines. The primary myoblast culture is time consuming, routine work (SOPs established) and require daily maintenance. Accurate records of stocks and arranging shipment to collaborators at University College London will also be needed. The role requires working closely with a postdoctoral researcher to differentiate myoblasts to myotubes for experimental purposes. You will provide a supporting role in experiments: western blotting, qRT-PCR, cell imaging, and functional studies using dissected muscle samples.Please note that due to Home Office visa requirements, this role is not suitable for first-time Skilled Worker visa applicants. Information on other visa options is available via the Government’s Work in the UK page.What we offer in return
And much more!If you are looking for a cell culture laboratory-based role in an internationally recognised research group that combines the University research and NHS diagnostic team then apply today.To explore the post further or for any queries you may have, please contact:
Knock, Western Isles – London
Sat, 14 Dec 2024 07:56:40 GMT
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