Senior/Principal Laboratory Research Scientist – Treisman laboratory

Job title:

Senior/Principal Laboratory Research Scientist – Treisman laboratory

Company

Francis Crick Institute

Job description

Senior/Principal Laboratory Research Scientist – Treisman laboratoryReports to Richard Treisman, Principal Group leaderThis full-time position on Crick terms and conditions of employment is available immediately for a period of three years.This role is advertised as a Senior/Principal Laboratory Research Scientist to encourage candidates having a broad range of experience to apply. We will appoint at the appropriate level depending on the experience and qualifications of the successful candidate.SummaryThis position in the Treisman group will participate in a programme of experimental research under the guidance of the Group Leader, provide support to group research staff, and be responsible for laboratory management.Project summaryCell cycle entry and progression, differentiation, and cell adhesion and motility are major targets for intracellular signals generated by growth factors. Work in the group focusses on Rho and Ras signalling, major signalling pathways involved in these processes. Central interests are: (1) the transcriptional regulator SRF, its cognate cofactors, and the gene network that it controls; and (2) the RPEL family of G-actin binding regulatory proteins, which represent a unique interface between cytoskeletal dynamics and cell regulatory processes. We use a multidisciplinary approach, involving biochemistry, structural biology, molecular biology, cell biology and genomics, applied to both tissue culture and mouse cancer and immune models. See https://www.crick.ac.uk/richard-treisman.Selected lab publications:Fedoryshchak*, R.O., and Treisman, R. A new FRET-based sensor for measurement of cellular G-actin concentrations. Manuscript in preparation.Maurice, D., Costello, P., Diring, J., Gualdrini, F., Frederico, B., and Treisman, R. Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8+ T cells during infectious challenge. Nature Comms, revision submitted.Fedoryshchak*, R.O., Přechová*, M., Butler, A., Lee, R., O’Reilly, N., Flynn, H., Snijders, A.P, Eder, N., Ultanir, S., Mouilleron, S. Treisman, R. (2020) Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife 9:e61509. DOI:Diring, J., Mouilleron, S., McDonald, N.Q. and Treisman, R. (2019) Nat Cell Biol. 2019 Jul;21(7):845-855. doi: 10.1038/s41556-019-0337-y.Foster, C.T., Gualdrini, F., Treisman, R. (2017) Mutual dependence of the MRTF-SRF and YAP-TEAD pathways in cancer-associated fibroblasts is indirect and mediated by cytoskeletal dynamics. Genes and Development 31(23-24):2361-2375.Gualdrini, F., Esnault, C., Horswell, S., Stewart, A., Matthews, N., Treisman, R. (2016) SRF co-factors control the balance between cell proliferation and contractility. Mol Cell 64(6):1048-1061.Esnault, C., Stewart, A., East, P., Horswell, S., Mathews, N., Gualdrini, F., and Treisman, R. (2014) Rho-actin signalling to the MRTF coactivators dominates the immediate transcriptional response to serum in fibroblasts. Genes and Development 28: 943-58.ResponsibilitiesThese include but are not limited to:Undertake research projects, designing and executing experiments, working independently with strategic direction from the group leaderApply molecular biology, biochemical, and cell biology techniques to cultured cell and mouse models, and train other group members in use of these techniquesProvide experimental support to other group members’ research projects according to need assessed by the group leaderTrain and supervise other group members as required in techniques, lab operations and equipment useProvide technical input for the development, refinement and troubleshooting of relevant methodologiesOrganisation of the laboratory including oversight of laboratory mouse, cell and reagent databases, routine maintenance of laboratory equipment, budget and allocation of resourcesParticipate fully in the group’s academic activities, including journal clubs, group meetings, scientific presentations, seminars and international conferencesExperience and SkillsThe post holder should be committed to the Crick core Crick values, and be bold, open, and collegial, in addition to the skills and qualifications:Essential – experimentalPhD (or equivalent experience) and postdoctoral experience in biochemistry, molecular biology or cell biologyKnowledge and understanding of molecular biology, gene expression, and signallingExperience and facility with DNA manipulationExperience in cell biology, including fluorescence microscopy, live cell imagingWillingness to learn new experimental skillsAbility to perform experimental analyses and data write upEssential – personalEffective written and verbal communication skillsAbility to communicate with others where English is not the first languageExperience of contributing effectively to the work of a teamGood organisational skills, ability to work independently and to prioritise workloadDesirableMouse tumour biology and transplantation techniquesExperience in biochemistry and protein purificationExperience analysing mass spectrometry dataExperience in gene manipulation using CRISPR technologyExperience facilitating interdisciplinary collaborationsExperience in laboratory managementExperience in mentoring lab members, including graduate studentsFind out what benefits the Crick has to offer:For more information on our great pay and benefits package please click here:Equality, Diversity & Inclusion:We welcome applications from all backgrounds. We are committed to providing equal employment opportunities, regardless of ethnicity, nationality, gender, sexual orientation, gender identity, religion, pregnancy, age, disability, or civil partnership, marital or family status. We particularly welcome applications from people who are Minority Ethnic as they are currently underrepresented in the Crick at this level.Diversity is essential to excellence in scientific endeavour. It increases breadth and perspective, leading to more innovation and creativity. We want the Crick to be a place where everyone feels valued and where diversity is celebrated and seen as part of the foundation for our Institute’s success.The Crick is committed to creating equality of opportunity and promoting diversity and inclusivity. We all share in the responsibility to actively promote dignity, respect, inclusivity and equal treatment and it is our aim to ensure that these principles are reflected and implemented in all strategies, policies and practices.Read more on our website:

Expected salary

Location

London

Job date

Wed, 24 Jul 2024 22:46:13 GMT

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